Leica extends multiphoton microscopy with integrated OPO
24 Jan 2011
Leica Microsystems has effectively matched the Leica
TCS SP5 MP multiphoton microscope with a fully integrated OPO (optical
parametric oscillator) system.
The integration of the Coherent Chameleon Compact OPO with the
Leica TCS SP5 MP confocal and multiphoton microscope allows
observation of fast dynamic processes in living cells with deep
penetration at high resolution with one integrated microscope
Full integration of the OPO within the software control of the
multiphoton system greatly facilitates research as it enables the
user to precisely adjust the excitation sources as well as all other
imaging settings within the same software. This makes the Leica
TCS SP5 MP with OPO a powerful tool for in vivo imaging.
The Leica TCS SP5 MP with OPO extends the choice of excitation
wavelengths from formerly up to 1080 nm to now up to 1300 nm. The
user can conveniently select between OPO and standard MP
excitation wavelengths and has the choice of simultaneous or
sequential excitation with the OPO and MP laser together.
Tubulin. Multiphoton excitation of Atto 647N at
The broad range of excitation wavelengths allows for multiphoton
excitation of dyes and cellular auto fluorescence from the visible
up to the red range of the spectrum. This allows the researcher to
use red dyes such as mCherry, DsRed2, mRFP, TexasRed and Alexa660
for multiphoton microscopy. Using dyes at longer excitation
wavelengths is less harmful to the specimen and decreases the amount
of scattered light. This allows deeper penetration into very thick
samples like thick tissue sections and whole mount preparations of
The addition of the Leica
OPO solution to the Leica TCS family greatly extends the range of
multiphoton applications with respect to multiphoton excitation in
NIR and IR, viability of the samples as well as penetration depth.