TruSeq SBS sequencing kit for genome analyzer
10 August 2010
Illumina, Inc. (NASDAQ:ILMN) has launched its TruSeq SBS
Sequencing kit that will enable high-quality paired end reads of 150
base pairs (bp), increase the output of high-quality base calls, and
enhance throughput to greater than 95 Gigabases (Gb) of high-quality
base pairs per run on Illumina’s Genome Analyzer (GA) sequencing system.
The kit is the first in a new series that will advance data quality,
ease of use and economical sequencing on the company’s sequencing
platforms. Individual products within the family of TruSeq reagents
will be specifically formulated for each Illumina sequencing
platform to best leverage the features of that system to provide the
highest yield and quality of data.
“At NCGR, we’re using TruSeq SBS chemistry to routinely generate
over 85% Q30 data for our 100 bp runs, which is a dramatic
improvement in both accuracy and output,” said Gregory May,
President and Chief Operating Officer at the National Center for
Genome Resources. “In addition, we’re now able to perform paired end
reads up to 150 bp on the Genome Analyzer, which will help us
greatly in both our de novo sequencing studies as well as our
ongoing agriculture sequencing projects.”
Coupled with advances in algorithms, the TruSeq SBS Sequencing
kit will enable researchers to increase the yield of Q30 base calls
and generate approximately 500 million perfect reads per 2x100 bp
run, setting a new standard for output of high-quality data from the
GA. The yield of perfect reads is an important indicator of a
system’s overall quality and helps greatly in mapping accuracy,
yield and overall coverage of a genome.
“The TruSeq family of reagents is the newest in a long line of
sample preparation and chemistry advancements focused on enhancing
ease of use, data quality and economical sequencing,” said Christian
Henry, Senior Vice President and General Manager of Life Sciences at
“The TruSeq SBS Sequencing kit will provide researchers with even
greater accuracy on the Genome Analyzer to generate data with higher
mapping rates as well as lower false positive and negative rates.
These improvements will materially enhance researchers’ ability to
paint the truest picture of the genome.”
Illumina says it intends to release the TruSeq SBS Sequencing kit
with TruSeq Cluster Generation kits and TruSeq Sample Preparation
kits for both DNA and RNA applications on the GA later this year.