High content screening automation for Leica confocal microscopes
1 June 2010
Leica Microsystems has released the the Leica HCS A, a
high-content screening automation package for confocal research
Combining the strength of high-resolution point scanners with the
new LAS AF Matrix M3 software platform, it delivers a flexible tool
for automated imaging in multi dimensions. It enables researchers to
customize Leica confocal microscopes according to actual
requirements in life science research laboratories.
Basic automation like mosaicing of tissue slides or multiwell
plate screening in 2D and 3D are part of the starting packages. More
sophisticated demands are satisfied by intelligent microscopy: five
new auto foci, single object tracking or automated control of water
objectives always deliver excellent results.
Storage and analysis is as important as generating thousands of
images, therefore the new export formats are compatible with the
Open Microscopy Environment image file format for biological image
data, OME.TIFF. Standard TIFF-files can be read by any modern image
analysis software, too.
Leica Microsystems developed the Computer Aided Microscopy
(CAM) interface to enable customisation of the microscope. By remote
control of the imaging process, the detection of rare events such as
mitosis phases is possible. Sophisticated algorithms can analyze
images on the fly and report the target coordinates to the
instrument via the programming interface CAM. Immediately, the
pre-scan switches to high-resolution zoom-in mode and starts to
image the target cells directly, offering excellent perspectives for
a new type of experiments.
Leica HCS A is designed for the core confocal platform systems.
Users can choose between the Leica TCS SP5 broadband confocal, the
Leica TCS LSI to visualize specimen from micro to macro scale, or
the Leica TCS SPE, known for ease of use and robustness.