Leica releases new digital camera for fluorescence applications
17 December 2009
The detection and documentation of low light fluorescence
signals in live cell experiments is a particular challenge for digital
cameras. To meet this demand, Leica Microsystems has added the new Leica
DFC345 FX to its portfolio of powerful digital cameras.
In addition to combining high sensitivity with high resolution, this new
camera features a fast image capture rate and a broad dynamic range,
making it ideal for multidimensional live cell observation under low
The two-megapixel CCD sensor (pixel size 4.4 µm) guarantees excellent
resolution of detail. The high dynamic range allows the acquisition of
multicolour images in one shot without time-consuming overlay. There is
a choice of 40 MHz pixel clock rate for fast image readout or a 20 MHz
pixel clock rate, ensuring low noise for top picture quality.
In low light conditions, photoelectric components have to be cooled
so that the camera can produce noise-free pictures during long exposure
That’s why the Leica DFC345 FX is equipped with one-stage Peltier
cooling for effective suppression of background noise. This, combined
with the high resolution of detail, produces brilliant images rich in
contrast — even for weak fluorescence signals.
Fully integrated for versatile application potential The FireWire-B
interface allows simple and reliable transfer of data to the computer
without an additional power supply. The Leica DFC345 FX is fully
integrated into the software platforms of Leica Microsystems: the
modular Leica Application Suite (LAS) for simple fluorescence
documentation and image archiving, the user-friendly LAS AF for
sophisticated, high speed fluorescence applications and Leica MM AF
(powered by MetaMorph) for demanding analysis.