Helicos BioSciences releases transcriptome sequencing (RNA-Seq)
datasets on open access website
23 April 2009
Helicos BioSciences (Nasdaq:HLCS) has released transcriptome
sequencing (RNA-Seq) datasets on the HeliSphere Technology Center, the
company’s open access website for sharing Helicos datasets and
bioinformatics software tools. Helicos’s amplification and ligation-free
approach virtually eliminates the creation of false-positive splicing
and fusion events, frequently observed in other RNA-Seq datasets.
Helicos RNA-Seq represents a powerful application for the Helicos
Genetic Analysis System by providing researchers with the ability to
obtain deep sequence coverage of the transcriptome for the elucidation
of novel transcripts, novel splice forms, and allele-specific expression
patterns, without the biases, artifacts and complexity introduced by
amplification and ligation. RNA-Seq was performed using polyadenylated
RNA from human brain and liver tissues, resulting in the generation of
~16 million aligned reads per channel for each sample.
With the high number of reads per channel and an extremely simple
sample preparation process, the Helicos platform provides the most
comprehensive view of the transcriptome for the lowest cost, allowing
the analysis of many samples in a single 50-channel run of the HeliScope™
Single Molecule Sequencer.
“Our collaborators have already made significant discoveries with the
results of Helicos RNA-Seq and are appreciating the benefits of
analyzing the transcriptome using the True Single Molecule Sequencing
approach, without ligation or amplification. We are excited to now
provide our customers with the methods to perform this analysis
themselves.” said Steve Lombardi, President of Helicos.
The amplification/ligation-free Helicos RNA-Seq protocol has been
released to a limited set of customers and is expected to be
commercialized later this year.
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