High-speed, high sensitivity camera for fluorescence imaging
13 July 2006
The new Nikon EMCCD Monochrome Digital Camera (DQC-FS) can
capture exceptionally low-level fluorescence and TIRF images more easily
than before. The new DQC-FS achieves single-photon detection
sensitivity typical of intensified or electron-bombarded CCDs but at a much
lower cost. It also offers higher resolution than conventional intensified
camera amplifies low-light-level signals above the CCD read noise by
employing unique electron multiplying CCD technology. This makes it ideal
for high speed and high sensitivity applications, such as molecular
fluorescence, high speed calcium and live cell fluorescence protein imaging.
The camera can also be used with LiveScan Swept Field Confocal systems and
for micro spectroscopy.
Incorporating a back-illuminated frame transferred
CCD, the camera produces a frame rate of 30 full frames per second at 10MHz,
giving it the ability to record specimen data at high temporal frequency and
high dynamic range using a 16-bit digitiser.
"Capturing images in low
light situations has become increasingly important in many areas of
research, and therefore the need for high quality digital imaging and
functionality is growing. With this in mind, Nikon’s technical experts
designed the novel DQC-FS to provide researchers with the most sophisticated
and light-sensitive technology available for some of the most demanding
digital imaging applications," said Chay Keogh, Nikon Instruments UK.
DQC-FS has the added benefit of including Nikon’s new NIS-Elements F
software. NIS-Elements incorporates advanced acquisition control of the
DQC-FS, including changing the digitisation rate, full binning control, and
full gain controls that allow users to utilise all of the camera's features.
The software also features basic image capture capability for every Nikon
automated microscope, as well as many third-party devices. Optional
NIS-Elements AR software extends the quantitative analysis virtues of the