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Biopharmaceutical Expression Systems and Genetic Engineering Technologies

Current and Future Manufacturing Platforms

Publication Date: November 2008
Publisher: BioPlan Associates
Pages: 315

Price £280.00
approximately: $418 | €334

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Summary

New expression systems and recent improvements available for current systems have the potential to revolutionize the biopharmaceutical industry! Lower costs and efficiencies of newer expression systems are driving these changes. This book provides you with the information you need to make the shift to increased productivity. As reflected by currently marketed products, since the advent of genetic engineering in the 1970s, there has been little basic change in the technologies used for commercial-scale manufacture of biopharmaceutical products.

Nearly all current products are manufactured using much the same old, familiar technologies — primarily using Esherichia coli (E. coli bacterium), Chinese hamster ovary (CHO) cells and the yeast Saccharomyces cerevisiae (S. cerevisiae) as hosts — technologies invented in the 1970s and commercialized in the 1980s.

Who Should Read this Book

Large and small drug innovators, CMOs, biogenerics companies; those seeking to develop new licensing/income streams; innovators dreaming of starting a new company, or people simply looking for a good investment. There are ample opportunities to get involved now, at the early stages of technology licensing. Find technological advances ready for adoption, adaptation and development, providing opportunities for process improvements and profit.

Benefits

Locate novel technologies, and contact innovators! Most major large (bio)pharmaceutical companies have made moves in this area, with some paying millions of dollars to acquire companies whose primary assets are new manufacturing platforms: ~$400 million--Merck & Co. acquired GlyoFi, for the manufacture of antibodies with human-like and modifiable glycosylation using the yeast Pichia pastoris; ~$56.5 million, Hoffmann-La Roche acquired Therapeutic Human Polyclonals Inc., a developer of methods for manufacture of polyclonal humanized antibodies in transgenic rabbits.

Opportunities for profit also exist:

Consider past technology licensing, e.g., Columbia University took in nearly $370 million over 17 years (average over $20 million/year) from licensing of co- transformation technology at less than a 1% royalty; Stanford University and the University of California each took in over $200 million from licensing of the Cohen-Boyer patents.

Why a Directory?

Access to the most important new technologies. Assess your commitment to your current systems. Consider how to implement total systems/platforms. Increase product yields and associated reductions in manufacturing costs.

Coverage

This book is designed to be the single most informative source concerning commercial biopharmaceutical product manufacturing-related expression systems and basic engineering technologies, with emphasis on those currently used for biopharmaceutical manufacture and those available for commercial licensing for this purpose; conduct further research and/or contact technology licensing sources.

Save considerable time and effort

Find technologies relevant to your interests. Coverage concentrates on host cells/organisms, basic genetic engineering methods, recombinant constructs and the many technologies available to enable or improve expression of desired proteins, including glycoproteins and antibodies. This directory concentrates on the core genetic materials (e.g., host cell lines and organisms) and related methods and materials, e.g., vectors, promoters, selection and amplification methods, chaperones, etc., useful for commercial-scale biopharmaceutical products.

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Content

  • Cell-free systems
    • 100 Cell-free expression, ATP regeneration system
    • 101 Cell-Free Protein Synthesis (Cell-Free)
    • 102 Large ribosomal subunit proteins, E. coli - cell-free systems
  • Broad, cross- or multi-platform technologies
    • 103 In vivo linearization; InVoLin; Meganuclease Recombination System (MRS) - improved transformation
    • 104 Vectors, site-specific recombinase assembly - eukaryotes
    • 105 Glycosylation, mammalian [Generic entry]
    • 106 Aminoglycoside phosphotransferase marker; Neomycin phosphotransferase I (nptI) marker; Geneticin (G-418) selection - universal
    • 107 Cellulose binding domain (CBD) fusion protein affinity tags; pET-CBD - universal
    • 108 Controllable Self-Cleaving Intein Derivative; IMPACT-CN; pH-sensitive self-cleaving fusion protein affinity purification tag - universal
    • 109 Cotransformation, Columbia University - eukaryotes, selection
    • 110 Cre-lox mediated in vitro recombination; Cyclization Recombination/locus of X-over P1; site-specific recombination (SSR) - universal; genetic recombination
    • 111 deltaPhase; Elastin-like polypeptide (ELP) fusion protein tags - chromatography-free purification; universal
    • 112 Directed Nuclease Editor (DNE); Meganuclease Design - universal
    • 113 Dual expression vectors; Dual Affinity ReTargeting (DART) - antibodies; bacteria and mammalian cells
    • 114 Expression enhancers, oligonucleotides - universal
    • 115 Expression factory; baculoworkstation - automated parallel expression
    • 116 Fusion protein expression systems; Affinity purification tags and chaperones - universal
    • 117 Fusion proteins, self-cleaving, pH-sensitive - universal
    • 118 Glutathione-S-transferase (GST) fusion protein affinity tags; pGEX vectors
    • 119 GUS reporter system (beta-glucuronidase); GUS control vector - prokaryotes; plant cells; mammalian cells, non-vertebrate
    • 120 Heat shock Promoters (HSPs); HSP chaperones
    • 121 His tags; Poly-Histidine fusion affinity tag technology; 6xHistidine-tag - univeral; purification tags
    • 122 His-tags; Poly-Histidine fusion affinity tag technology - univeral; purification tags
    • 123 Hybridomas (Khler and Milstein) - monoclonal antibodies
    • 124 Lambda recombination protein; Homologous recombination
    • 125 Meganuclease Recombination System (MRS)/I-SceI; homologous recombination - universal genetic recombination
    • 126 Mucin promoters; IIM14 and IIM22 - vector enhancers
    • 127 New Cabilly; Cabilly-Boss; Monoclonal antibody 2-chains expression - universal
    • 128 phCMV vectors; GenePORTER - universal
    • 129 Poly(A) polymerase
    • 130 Promoters
    • 131 Recombinant DNA; Protein Expression; Cohen-Boyer
    • 132 Reconstituting chemically orthogonal directed engineering (ReCODE) - Unnatural amino acids; UAAs; E. coli; yeasts; mammalian cells
    • 133 Selectable markers; Selection of transformed cells; Reporter genes
    • 134 Small Ubiquitin-like Modifier (SUMO) fusion protein tags; Split SUMOpro System; Ubl-specific protease - universal
    • 135 Strep-tag affinity fusion protein tag; Strep-Tactin purification - universal
    • 136 Super core promoters; SCP1; Core promoters - the strongest [promoters] ever made
    • 137 TAGZyme; dipeptidyl aminopeptidase I (DPPI; DAPase Enzyme; cathepsin C) - cleavage of His tags; universal
    • 138 TEV Protease; Tobacco Etch Virus (TEV) NIa protease - cleavage of fusion
    • protein tags; universal
    • 139 Transfection - universal
    • 140 Translation Engineering expression; CODA; Computationally Optimized DNA Assembly; Hot Rod Genes; Controlled Ribosomal Pausing - universal
    • 141 White collar complex (WCC) promoter; UV light induction - universal
    • 142 Minos transposon cell transformation - insect larvae; also eukaryotes
    • 143 Coconut Express cell-free translation - plants
  • Prokayotes
    • 144 Bacterial cell expression technology (BCE); araB promoter - antibody fragments; E. coli; prokaryotes
    • 145 Subtilisin (psub) fusion protein tag expression; Profinity eXact expression - E. coli; bacteria; yeasts; CHO cells
    • 146 Tetracycline-induced expression repression - prokaryotes
  • Bacteria
    • 147 Bacillus megaterium expression - E. coli alternative
    • 148 Bacillus subtilis, super-oxidizing strains; Thiol-disulfide oxidoreductases (TDORs); Thioredoxin A (TrxA) depletion - disulfide bridge optimization
    • 149 Bacterial artificial chromosome (BAC) expression; pBAC vectors - bacteria
    • 150 Caulobacter crescentus expression; PurePro Caulobacter Expression System - Caulobacter bacteria hosts
    • 151 Clostridium Expression System; NTNH promoter from Clostridium botulinum
    • 152 Flavobacterum heparnum expresson - glycoprotens
    • 153 Lactococcus lacts htrA- expresson - protease depleton
    • 154 Lactose (lac) promoters; Isopropyl-beta-galactosdase (IPTG) nducton - bactera
    • 155 NIsn-Controlled gene Expresson (NICE); Lactococcus lacts expresson; nsA promoter - antbotc selecton
    • 156 P170 Expresson System;Lactococcus lacts expresson
    • 157 Pfenex Expresson; Pseudomonas fluorescens bovar I (MB101) - E. col alternatve
    • 158 Plasmds stablzaton
    • 159 Pseudoalteromonas haloplankts TAC125 (PhTAC125) - cold expresson
    • 160 Quas-synthetc vectors; Synthetc gene sequences n vectors - bactera
    • 161 Ralstona eutropha expresson; Alcalgenes eutrophus expresson
    • 162 Rhodosprllum rubrum (bacteral) expresson - membrane protens
    • 163 Staphylococcus carnosus expreson
    • 164 Subtln Regulated Gene Expresson; SURE competency - B. subtls
    • 165 E. col expresson/vectors
    • 166 CASCADE expresson; pALEX1 plasmds - E. col
    • 167 Cholne-bndng fuson affnty tags - bactera
    • 168 Clean Genome E. col; Strpped-down E. col
    • 169 GroEL,GroES chaperones; Chaperonns - proper foldng; unversal; E. col
    • 170 Methylobacterum extorquens (bacterum) expresson
    • 171 CANGENUS; Streptomyces (lvdans and grseus) expresson
    • 172 Saccharomyces cerevsae expresson
    • 173 Streptomyces statonary phase expresson; SPE system; Streptomyces vectors; Secreted Protease Producton (SPP) System
    • 174 Streptomycetes hyper-nducble expresson; PntA-NtR system; Caprolactam nducton - Streptomycetes
  • Yeasts
    • 175 ApoLfe Yeast Expresson; S. cerevsae Twn Cassette Plasmds - antbodes n yeast
    • 176 Arxula adennvorans expresson - alternatve yeast
    • 177 Chrysosporum lucknowense expresson; C1 Express Hyperproducng Proten Expresson System - fung
    • 178 CoMed system; Unversal Yeast vectors; pCoMed vectors; Arxula adennvorans-derved TEF1 promoter
    • 179 Fungal expresson systems
    • 180 GlycoF technology; Next Generaton Botherapeutcs - Pcha pastors; yeasts; glycosylaton; antbodes
    • 181 Hansenula expresson (yeast)
    • 182 Hansenula polymorpha (yeast) expresson - E. col alternatve
    • 183 Kluyveromyces lacts expresson; pKLAC1; Acetamdase/Acetamde selecton; K. lacts GG799 - yeast
    • 184 NeuBIOS expresson; Neurospora crassa expresson; NeuKARYON - flamentous fung; glycosylaton; Cablly-Boss workaround
    • 185 Neurospora expresson; cotA promoter - fung
    • 186 Ophostoma expresson - ascomycetes fung
    • 187 Yeast expresson systems
    • 188 Zygosaccharomyces bal expresson; Zbleu2 stran - yeast
    • 189 EASYEAST; Saccharomyces cerevsae strans - easy proten release
    • 190 Yeast cell lnes and vectors; Saccharomyces cerevsae cell lnes - proper foldng and glycosylaton
    • 191 Pcha pastors expresson
    • 192 VelocMab; EESYR expresson system; FASTR cell lnes - CHO expresson optmzaton; antbodes
  • Plants
    • 193 Plastd Transformaton; Translaton-based vectors (TBV) - plants
    • 194 Chlamydomonas renhardt chloroplast expresson; promoter - algae, sngle-cell
    • 195 Chlamydomonas renhardt expresson - algae, sngle-cell
    • 196 Drosophla Expresson System (DES) - nsect cell culture
    • 197 Streptomyces lvdans
  • Eukaryotes
    • 198 Antbotc nducble promoters - eukaryotes
    • 199 AttSte recombnases - precse gene nserton; eukaryotes
    • 200 Bovne growth hormone (bGH) polyadenylaton sequence - eukaryotes; expresson enhancement
    • 201 Expresson enhancers; Copy number ncrease - eukaryotc cells
    • 202 Homologous Recombnaton; Knock-out/knock-n anmals and cells
    • 203 Internal Rbosome Entry Sequences (IRES) RNA translaton enhancers; pCITE vectors; Cardovrus 2A IRES; pIRES - eukaryotes
    • 204 Lght-swtchable promoters
    • 205 Tetracyclne (Tc; Tet) Expresson Systems - eukaryotes
    • 206 Znc fnger DNA-bndng protens (ZFPs); ZFP Transcrpton Factors - gene modfcaton; mammalan cells; plant cells
  • Protozoa
    • 207 Clate Performance Expresson System; CIPEX system; Tetrahymena (protozoa) expresson
    • 208 LEXSY; Leshmana tarentolae expresson system - protozoa
    • 209 Perknsus marnus expresson - protozoa express large protens
    • 210 TetraExpress; Tetrahymena (protozoan) expresson
    • 211 Tetrahymena thermophla (protozoan) expresson
  • Anmals, msc.
    • 212 Mlk proten promoter - protens n mlk of transgenc anmals
    • 213 Shrmp expresson; Penaeus stylrostrs expresson; Taura Syndrome Vrus (TSV) IRES vectors - glycoprotens; antbodes
    • 214 Transgenc rabbts - humanzed polyclonal antbodes
  • Mammalan
    • 215 AmProten vectors - stongest mammalan vector set; unversal
    • 216 Ant-apoptoss expresson system; BCL-xL or BCL-2 expressng cell lnes - CHO, NS0, BHK, SP2/0-Ag14
    • 217 Autocatalytc cleavage stes; Mengo vrus vectors; Scsson cassettes - mammalan cells
    • 218 Cell adheson optmzaton; cdkl3, sat7e, and lama4 genes - antbody-expressng cells
    • 219 CMV (human) promoter; Cytomegalovrus promoter; Complete Control Inducble Mammalan Expresson System - mammalan cells
    • 220 Cumate gene-swtch; Q-mate Inducble Expresson - mammalan cells
    • 221 Dhydrofolate reductase (DHFR) System - selectable marker/amplfcaton; CHO and NS0 cells
    • 222 Flp-In expresson system; FLP-Medated Gene Modfcaton n Mammalan Cells; FLP recombnase - mammalan cells
    • 223 Gene-Actvated (GA) expresson, n vvo - mammalan cells
    • 224 Glutamne synthetase (GS) System - NS0, CHO, mammalan cells
    • 225 GPEx Gene Product Expresson Technology - mammalan; CHO
    • 226 MARtech; Matrx Attachment Regons; Scaffold Attachment Regons; SARs - mammalan cells
    • 227 RheoSwtch Mammalan Inducble Expresson System; RheoSwtch Lgand RSL1 promoter; Ecdysone receptor nducton - mammalan cells; adjustable expresson
    • 228 Selexs Genetc Elements (SGEs) - mammalan cell lnes
    • 229 STablzng Ant-Represson; STAR elements - expresson enhancement; mamalan cells
    • 230 Ubqutous chromatn openng element (UCOE) expresson - mammalan cells
    • 231 Whey acdc proten (WAP) mlk promoters - mammals
  • Chnese hamster ovary (CHO) cells
    • 232 ACE Expresson System - MAb-expressng CHO cells lnes
    • 233 Boehrnger Ingelhem Hgh Expresson System (BI HEX); CHO-DG44
    • 234 CHO cell lne (Puck); CHO-K1 cell lne
    • 235 CHO DG44 cells, DHFR-; CHO-DG44; DUK-XB11; CHO K1 DUX B11 (DHFR-) cells; Dhydrofolate reductase selecton/amplfcaton, CHO cells
    • 236 CHO SSF cell lnes - adherent CHO cells; proten-free meda
    • 237 CHO Supercell; Targeted transfecton; CHO DG44 cell lne
    • 238 CHOZn CHO DG44 cell lnes - antbodes
    • 239 GS-CHO Proten Free System; CHOK1SV cell lnes - proten-free meda
    • 240 Sympress expresson; Human polyclonal antbodes (rpAB) - CHO cells
    • 241 Baby hamster kdney (BHK) 21 cells; ATCC CCL 10
  • Hybrdomas
    • 242 Human MORPHODOMA; Morphogencs; Hypermutaton; PMS2 gene screenng, modfcaton - human monoclonal antbodes from hybrdomas
    • 243 Cell-free system - glycoprotens; hybrdoma
    • 244 Ex-Cell EBx expresson; EBx cells; Chcken embryonc stem cells; Chcken EBx cells; Duck EBx cells
  • Chckens/Poultry
    • 245 Chcken egg expresson; Transgenc anmals
    • 246 Chcken rmordal germ cells (PGCs) - Human protens/monoclonal antbodes n chcken eggs
    • 247 OVA System; Avan Transgenc Bomanufacturng - chcken eggs expressson
    • 248 Transgenc poultry; avan transgeness and nuclear transfer - protens n chcken eggs
    • 249 Wndowng Technology - transgencs avans/chckens
  • Human
    • 250 CEVEC Amnocyte Producton (CAP) expresson; Human amnocytes, mmortalzed
    • 251 bcl-2 (p21) overexpressng NS0 cell lnes - NS06A1(100)3 cell lne - antagonze apoptoss; Mabs
    • 252 Cell fuson, NS0 cells - antbodes
    • 253 Cholesterol/3-ketosterod reductase (p3-KSR) expresson - cholesterol selecton/nducton of NS0 cells
    • 254 GlycoExpress human cell lnes; NM-F9 cell lne - glycolyss
    • 255 Human prmary preB lymphocytes; HupreB cells - human monoclonal antbodes
    • 256 NS0 murne myeloma cell lne
    • 257 NS0-PFCF cells; NS0 cells, proten- and cholesterol-free - antbodes
    • 258 PER.C6 expresson; Extreme densty (XD) Technology - glycoprotens; antbodes
    • 259 Retrotransposon vectors - transgenc avan/chcken cell culture
    • 260 Sp2/0-Ag14 cells - proten-free meda; antbodes
    • 261 HEK 293 cell lne, proten- and peptde-free (Hektor G) meda; human embryonc kdney (HEK 293) cell lne -
    • 262 HEK 293 expresson
    • 263 HEK 293 expresson
    • 264 HEK293 cell lnes
    • 265 HEK293SFE cell lne
    • 266 HKB-11 (HKB11) expresson; Hybrd of kdney and B cells - HEK-293 alternatve
  • Plants
    • 267 Agrobacterum tumefacens; T plasmds - transgenc plants
    • 268 Antbotc resstance markers - plants
    • 269 Chloroplast expresson - plants
    • 270 Chloroplast Transformaton Technology (CTT) - plant cells
    • 271 Coupled regeneraton/ transformaton, plants
    • 272 GENEWARE expresson; Tobacco mosac vrus (TMV) vectors - tobacco; plants
    • 273 Glyco-Engneered Moss; Physcomtrella patens expresson; moss expresson promotng regons (MEPRs) - glycosylaton; antbodes
    • 274 BoLaunch expresson; Launch vectors - protens and Mabs n plants
    • 275 LEX System; Lemna (duckweed) expresson - algae, whole plants
    • 276 Nuclear transfer Cultured nner cell mass cells (CICM) - transgenc anmals; clonng from somatc cells
    • 277 Nuclear Transformaton Sute, plants
    • 278 Plant expresson - glycosylaton
    • 279 pPIPRA vectors - plants; publc doman
    • 280 ProCellEx Plant Expresson - plant cells; glycosylaton
    • 281 Profca expresson - transent expresson, plants
    • 282 RNA-dependent RNA polymerase (RdRP) - unversal
    • 283 Stratosome Bologcs System; Olbody expresson; Safflower plant seed expresson
    • 284 Super-mas Plant Gene Promoter; Gelvn promoter; (Aocs)3AmasPmas - plants
    • 285 TransBacter Gene Transfer System -plants; royalty-free
    • 286 Ubqutn lnkage doman - multple protens n transgenc plants
    • 287 Ubqutn plant promoters
    • 288 Zara technology; Proten body-nducng sequence (PBIS) fuson protens; Recombnant proten body-lke assembly (RPBLA); StorPro organelles (proten encapsulaton); Prolamn fuson protens - eukaryotes
    • 289 CleanGene plant transformaton
    • 290 Plastds (chloroplasts) expresson - plant cell culture
  • Insects
    • 291 Hgh Fve cell lne (BTI-TN-5B1-4, ATCC CRL 10859); Trchopulsa n cell lne - baculovrus host cells; nsect cell culture
    • 292 Insect cells glycosylaton
    • 293 Insect cells glycosylaton
    • 294 Insect cells/Baculovrus expresson systems; Baculovrus expresson vector systems (BEVS)
    • 295 PERLXpress; TRANSPILLAR larvae; Trchoplusa n larvae expresson - transformed caterpllars
    • 296 Trchoplusa n (cabbage looper) cell lnes; BTI-TN-MG1; ATCC CRL 10860; BTI-TN-5B1-4; ATCC CRL 10859
    • 297 Trchoplusa n (cabbage looper) cell lnes; H5CL-B and H5CL-F; BTI-TN-5B1-4-derved nsect cell lnes
    • 298 Baculovrus expresson vector systems (BEVS)
    • 299 InsectSelect Proten Expresson System - nsect cells; avod baculovruses
    • 300 Mmc Sf9 Insect Cells - mammalan-lke glycosylaton
    • 301 Polydnavrus vectors - nsect cells; baculovrus altneratve
    • 302 Pre-occluded Vrus (POV) baculovrus vectors; Insect cells, per os (oral) baculovrus nfecton
    • 303 Spodoptera frugperda Sf-21 cell lne - baculovrus host nsect cells
    • 304 Spodoptera frugperda Sf-9 cell lne - baculovrus host nsect cells
    • 305 NusA E. col fuson protens - eukaryotes; proten solublzaton
  • Other broad/unversal and older technologes
    • 306 Enteroknase - fuson proten cleavage
    • 307 Phage lambda promoters; PL promoter; PR promoter - E. col
    • 308 Phage T5 promoter
    • 309 WI-38 cell lne, Normal human fetal lung fbroblasts
    • 310 Alkalne Phosphatase; Calf Intestnal Alkalne Phosphatase (CIAP) - prevent vector recrcularzaton
    • 311 Benzonase; Serrata marcescens endonuclease - polynucleotdes breakdown
    • 312 DNA Lgase (E. col)
    • 313 Ste-drected mutageness
    • 314 T4 DNA Lgase
    • 315 T4 RNA Lgase
    • 316 Altogen transfecton; RNA gene slencng
  • Bactera/Prokaryotes
    • 317 Profuse vectors; Csperone chaperone fuson tags - E. col; Saccharomyces cerevsae
    • 318 Bacteral transcrpton promoters
    • 319 BresaGen fuson proten expresson - E. col
    • 320 Cold-Induced expresson - Bacllus subtls
    • 321 desA promoter, ron-regulated; DmdR repressors - Actnomycetes
    • 322 E. col. vectors; Mnt-Arc promoters; T1 and T2 rrnB rbosomal termnators - bactera
    • 323 pAVEway expresson - E. col and Pseudomonas
    • 324 pTAT-HA plasmds - E. col; bactera
    • 325 Twn-argnne translocaton (Tat) system; Tat nanomachne - proten foldng, then secreton; bactera
    • 326 VegI promoters - Bacllus subtls and E. col
    • 327 Xer-cse gene excson; Xer recombnases - Bacllus subtls
    • 328 Agrobacterum tumefacens RpoA co-expresson transcrptonal actvator - E. col
    • 329 Avdn affnty fuson proten affnty tags; Botn purfcaton; PnPont Xa Proten Purfcaton System - E. col
    • 330 Bogenercs manufacturng technology packages - E. col
    • 331 BL21(DE3) competent E. col cells
    • 332 C-LYTAG affnty fuson proten tag; Streptococcus pneumonae N-acetylmuramoyl-L-alanne amdase LytA - E. col; purfcaton
    • 333 Campylobacter jejun glycosylaton genes; OTase of C. jejun - glycosylaton; E. col
    • 334 Chaperone expresson plasmds; DnaK, DnaJ and GrpE chaperones - E. col
    • 335 cs-Actng Peptde chaperones - E. col
    • 336 Codon-Optmzed, Expresson-Ready E. col Clones
    • 337 Contnuous culture - bactera; E. col
    • 338 Dsulfde somerase coexpresson; DsbC and DsbG - E. col; dsulfde bonds and foldng
    • 339 Elastn-lke polypeptde (ELP) self-cleavng fuson proten tags - chromatography-free purfcaton; E. col
    • 340 ExpressProtect; p26, ScA, and alpha-crystalln-type fuson proten tags - E. col
    • 341 Hgh copy number plasmds; pBGP120 - E. col
    • 342 Hgh transformaton effcency (Hte) competency - E. col cells
    • 343 Hs-Patch ThoFuson System; pThoHs vector - E. col; fuson protens
    • 344 N(pro) fuson proten tag, self-cleavng; Swne fever vrus N(pro) autoproteolyss; EDDIE - E. col
    • 345 OverExpress C41(DE3) and C43(DE3) - E. col strans
    • 346 OxlT plasmds; Oxalate/Formate Exchange Proten - E. col
    • 347 pBR322 - E. col plasmd; antbotc selecton
    • 348 pCold vectors; Cold Shock Proten A (cspA) promoters - E. col, cold nducton
    • 349 pET Expresson System; T7 promoter; pET Drectonal TOPO Clonng; Champon pET Expresson Vectors; T7 RNA polymerase (T7 RNAP) - E. col
    • 350 pMAL Proten Fuson and Purfcaton System; Maltose bndng (MBP) fuson affnty tags; pMAL plasmds - antbody fragments; E. col
    • 351 Polyhydroxybutyrate (PHB) fuson tags, self-cleavng coexpressed wth affnty medum - E. col; unversal
    • 352 Red/ET recombnaton; ET clonng/ET recombnaton; GET recombnaton; Recombneerng; f Red-medated recombnaton; lambda-medated recombnaton - E. col
    • 353 Skp and DsbC chaperone fusons - E. col; secreton control
    • 354 Tac promoters - E. col
    • 355 Tryptophan (trp) promoters - E. col
    • 356 WACKER Secreton System; E. col K12-based secreton system - antbody fragments
    • 357 Flavvrus vectors; Kunjn replcon vectors - prokaryotes; Streptomyces; prokaryotes
    • 358 Streptomyces nducers
    • 359 Streptomyces lvdans strans; xysA promoters
  • Yeasts
    • 360 AlcoFree Yeasts; Saccharomyces cerevsae KOY.TM6* strans
    • 361 ALEU2 marker; AHSB4 promoter; Arxula adennvorans expresson
    • 362 Aspergllus nger expresson; A. nger A4 promoters - humanzed antbodes
    • 363 Aureobasdn A vectors (pAUR) - selectable marker n yeasts
    • 364 Calnexn chaperone - Hansenula polymorpha; yeasts
    • 365 Chtn synthase (CHS1), Yeast growth factor, chtn synthase (CHS1) - yeast promoter dscovery
    • 366 Estradol-dependent enhancer; Gal4-ER-VP16 - yeasts
    • 367 Formaldehyde dehydrogenase (FLD1) promoter; Formaldehyde selecton - Pcha pastors; yeasts
    • 368 GAPFL promoter; Glyceraldehyde-3-phosphate dehydrogenase-derved promoter - yeasts
    • 369 Gene Desgn, algorthmc - yeasts
    • 370 Hypermutable yeast
    • 371 PH05 promoter; Phosphate nducton -yeasts
    • 372 Vescular fuson factor 2 proten (Vff2p) enhancer - yeast; bactera; CHO cells
    • 373 Vescular fuson factor 2 proten (Vff2p) enhancer - yeasts
    • 374 Xplor Vector System, yeast optmzaton
    • 375 Antbody fragment expresson - Saccharomyces cerevsae
    • 376 Saccharomyces cerevsae, cold nducton
    • 377 Secreton Enhancer Vector System (SEVS); SecHancer vector - Saccharomyces cerevsae
    • 378 Mab Xpress Antbody Producton System; Pcha pastors - glycosylated antbodes
    • 379 Pcha expresson, rhamnose nducton
    • 380 Pcha GlycoSwtch System; Glycoswtch plamds - yeasts; glycosylaton
    • 381 Pcha pastors antbody expresson
    • 382 Pcha pastors AOX1 promoters
    • 383 Pcha pastors super yeast; Pcha pastors AOX1 promoters
    • 384 Amnoglycosde adenylyltransferase (aadA1) promoter - bactera; eukaryotes
    • 385 ColE1 plasmds, E. col - prolonged vablty
  • Mammals
    • 386 CMV/R Promoter - eukaryotes
    • 387 pggyBac transposon - eukaryotes; nsect cells
    • 388 Tax-nducble expresson; Bovne leukema vrus (BLV) promoter - mammalan cells
    • 389 BacMam; pBacMam vectors - mammalan vectors, baculovrus-based
    • 390 Calnexn, calretculn, Erp57, Hsp40, and Hsp70 chaperones - mammalan cells
    • 391 CCT promoters - mammalan cells
    • 392 ClonePxFL Selecton - antbodes, mammalan cells
    • 393 Hsp60, Hsp70, Hsp90, Hsp100 chaperones - mammalan cells
    • 394 IRF-1 estrogen receptor promoter; Estradol nducton - mammalan cells
    • 395 Osteoclast-assocated receptor (OSCAR) promoter - mammalan; CHO cells
    • 396 pAccAB vectors - antbodes; mammalan cells
    • 398 RP Shft; Senescence nducton; PACE Expresson Vector - mammalan cells; expresson enhancement; antbodes
    • 399 Semlk forest vrus (SFV) vectors - mammalan and nsect cells
  • Chnese Hamster Ovary (CHO) cells
    • 400 CHEFl expresson; CHO elongaton factor-la (EF-Ialpha) promoter - CHO cells
    • 401 CHO cells - antbodes; serum-free meda
    • 402 CHO cells dhfr RNA nterference; RNA slencng vectors - CHO cells
    • 403 CSL4S-342 CHO cells (CHO-K1 CSL4S-342)
    • 404 Osteoclast-assocated receptor (OSCAR) promoter - mammalan; CHO cells
    • 405 Pangen CHO expresson
    • 406 StableFast Bomanufacturng System; pBFdfhr.2 Expresson Vector -
    • CHO cells; antbodes
    • 407 Tandem Chmerc Antbody Expresson (TCAE) vectors; ANEX vectors; CHO cell lne TCAE 8 (ATCC 9119); Kozak sequences, mpared - antbodes
    • 408 UTR Clone Generaton; UTRtech; Cell Factores; Gaussa lucferase sgnal peptdes - Mabs supersecreton; CHO cells
    • 409 DNA mcronjecton - transgenc anmals, chckens HEK-293 cells
    • 410 293ST-3F6 cell lne; HEK-293 adapted to SFM
    • 411 CRE-nducble expresson; cyclc AMP response elements (CREs) -
  • HEK-293 cells
    • 412 HEK-293 expresson
    • 413 HEK-293 expresson
    • 414 pTT vectors for HEK-293E cells
  • Plants
    • 415 Magnfecton; magnICON; Transgene Operatng System (TOS) - antbodes; plants and plant cells
    • 416 MARs PLUS; Matrx attachment regons PLUS - plants
    • 417 Concert Plant-Cell-Produced system - tobacco plant cell culture
    • 418 Phyton plant cell fermentaton
    • 419 ExpressTec expresson; ExpressPro; ExpressMab - rce and barley; antbodes
  • Insects
    • 420 AcMNPV p35 apoptoss nhbton; Sf9P35AcV5-1 and Sf9P35AcV5-3 - nsect cells, apoptoss resstance
    • 421 AF 99 nsect cell lne
    • 422 BL-Sf-21AE-Cl 3 cell lne - Insect cell lnes, baculovrus hosts
    • 423 Cre/loxP Recombnaton-Medated Cassette Exchange (Cre/loxP RMCE) - Drosophla (mosqutos)
    • 424 Drosophla expresson
    • 425 Drosophla melanogaster S2 cells; Drosophla-SFM.D.Mel-2 Cells; Schneder S2 Drosophla cells; S2 cells, SFM - nsect cell culture
    • 426 IE-1 (BmNPV 1.2 kb fragment) promoters; Bombyx mor actn promoters - nsect cells
    • 427 Insect cell lnes - baculovrus hosts
    • 428 Insect cells, per os (oral) baculovrus nfecton
    • 429 Lymantra dapar nucleopolyhedrovrus and L. dspar 652Y (Ld652Y) cell lnes - baculovrus host cells
    • 430 pIEx baculovrus vectors; hr5 enhancer; e1 mmedate early promoter - nsect cells; avod baculovrus pathogencty
    • 431 Rhopalosphum pad vrus Internal Rbosome Entry Sequence (IRES); Pcorna-lke vrus IRES; Drosophla IRES - nsect cells; plant cells
    • 432 Tn PRO; Trchoplusa n cell lne
    • 433 BAC-TO-BAC Baculovrus Expresson System; baculovrus shuttle vectors; bacmds - nsect vectors produced n E. col
    • 434 BacTen System; p10 promoter vectors - nsect cells
    • 435 Baculovrus vectors and promoters - glycosylaton
    • 436 BestBac vectors; Autographa calfornca nuclear polyhedross vrus (AcNPV) vectors - nsect cells
    • 437 Drosophla salyltransferases - nsect cells; glycosylaton
    • 438 Sapphre baculovrus expresson - dsulfde bond formaton
    • 439 Vankyrn enhanced baculovrus expresson vector system (VE-BEVS); Vankyrn-enhanced cell lnes (VE-CL)
  • Indexes
    • Company/Organzaton Index
    • Subject Index
    • Prmary Host/Organsm Index

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